Zuccheri G., Samorì D. (Eds.). DNA Nanotechnology: Methods and Protocols

Springer, 2011. - 374 p. - Currently, nanotechnology is exposing the properties of DNA in unprecedented detail leading to new insights on the biological behavior and function of DNA. With the structural perfection of a self-assembling DNA nano-object, such as a DNA origami, it is clear how complex DNA is as a molecule, leading researchers to wonder how many different constructs could be designed and realized. DNA Nanotechnology: Methods and Protocols shows the procedures to follow in order to repeat methods that lead to such constructs or to the mastering of the characterization techniques used to study them. The chapters of this book are roughly divided into two parts: some cover the methods for preparing the nanostructures, from the rationale of the operations to the techniques for their handling, while other chapters deal more directly with advanced instrumental techniques that can manipulate and characterize molecules and nanostructures. Written in the highly successful Methods in Molecular Biology series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, DNA Nanotechnology: Methods and Protocols serves as an ideal guide to scientists of all backgrounds and aims to ignite interest and spur activity in this young and rapidly growing research field. Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the expertsSynthesis and Characterization of Self-assembled DNA Nanostructures
Prools for Self-assembly and Imaging of DNA Nanostructures
Self-Assembly of Metal-DNA Triangles and DNA Nanotubes with Synthetic Junctions
DNA-Templated Pd Conductive Metallic Nanowires
A Method to Map Spatiotemporal pH Changes Inside Living Cells using a pH Triggered DNA Nanoswitch
Control of Helical Handedness in DNA and PNA Nanostructures
G-quartet, G-quadruplex, and G-wire Regulated by Chemical Stimuli
Preparation and Atomic Force Microscopy of Quadruplex DNA
Synthesis of Long DNA-based Nanowires
G-wire Synthesis and Modification with Gold-nanoparticle
Preparation of DNA Nanostructures with Repetitive Binding Motifs by Rolling Circle Amplification
Controlled Confinement of DNA at the Nanoscale: Nanofabrication and Surface Bio-functionalization
Templated Assembly of DNA Origami Gold Nanoparticle Arrays on Lithographically Patterned Surfaces
DNA-modified Single Crystal and Nanoporous Silicon
The Atomic Force Microscopy as a Lithographic Tool: Nanografting of DNA Nanostructures for Biosensing Applications
Trapping and Immobilization of DNA Molecules Between Nanoelectrodes
DNA Contour Length Measurements as a Tool for the Structural Analysis of DNA and Nucleoprotein Complexes
DNA Molecular Handles for Single Molecule Protein Folding Studies by Optical Tweezers
Optimal Practices for Surface-tethered Single Molecule Total Internal Reflection Fluorescence Resonance Energy Transfer Analysis
Engineering Mononucleosomes for Single-Pair FRET Experiments
Measuring DNA-Protein Binding Affinity on a Single Molecule Using Optical Tweezers
Modeling Nanopores for Sequencing DNA