Spilling K. (Eds.) Biofuels from Algae: Methods and Protocols

Springer, 2020. — 248 p. — (Methods in Molecular Biology 1980). — ISBN: 978-1-4939-9415-1.This volume provides a comprehensive overview of different ways to grow algae and the techniques used to start algal cultivation, monitor algal growth, environmental impact of growing algae, and various methods for characterizing the biomass. The chapters in this book discuss a range of topics such as step-by-step protocols on how to isolate and grow algae; descriptions on how to use fluorescence to estimate algal biomass and lipid content; and instructions on using advanced techniques to determine the carbohydrates and lipids in algal biomass. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Biofuels from Algae: Methods and Protocols is a valuable resource for novice and experienced researchers who want to learn more about this impactful and developing field.Large Scale Cultivation of Microalgae: Open and Closed Systems
Design and Analysis of Offshore Macroalgae Biorefineries
Basic Methods for Isolating and Culturing Microalgae
Measurement of Fluorescence for Monitoring Algal Growth and Health
Microalgae Lipid Staining with Fluorescent BODIPY Dye
Monitoring Microalgal Neutral Lipid Accumulation with Nile Red
Determining Inorganic and Organic Carbon
Determining Inorganic and Organic Nitrogen
Total Nitrogen Determination by a Spectrophotometric Method
Determining Inorganic and Organic Phosphorus
Determining Dissolved and Biogenic Silica
Life Cycle Assessment of Seaweed Cultivation Systems
Life-Cycle Assessment (LCA) Analysis of Algal Fuels
Metabolic Engineering of Microalgae for Biofuel Production
Analytical Grade Purification of Phycocyanin from Cyanobacteria
Complete Acid-Based Hydrolysis Assay for Carbohydrate Quantification in Seaweed: A Species-Specific Optimized Approach
Total Carbohydrate Content Determination of Microalgal Biomass by Acid Hydrolysis Followed by Spectrophotometry or Liquid Chromatography
Total Fatty Acid Content Determination of Whole Microalgal Biomass Using In Situ Transesterification
Liquid Chromatography-Mass Spectrometry (LC-MS)-Based Analysis of Molecular Lipids in Algae Samples
UPLC-ELSD Analysis of Algal Lipid Classes and Derivatization of Bound and Free Fatty Acids and Sterols for GC-MS Methods
Total Protein Content Determination of Microalgal Biomass by Elemental Nitrogen Analysis and a Dedicated Nitrogen-to-Protein Conversion Factor
Correction to: Determining Inorganic and Organic Nitrogen
Correction to: Determining Inorganic and Organic Phosphorus