Marcu L., French P.M.W., Elson D.S. (eds.) Fluorescence Lifetime Spectroscopy and Imaging: Principles and Applications in Biomedical Diagnostics

CRC Press, 2015. — 553 p.During the past two decades, there has been an increasing appreciation of the significant value that lifetime-based techniques can add to biomedical studies and applications of fluorescence. Bringing together perspectives of different research communities, Fluorescence Lifetime Spectroscopy and Imaging: Principles and Applications in Biomedical Diagnostics explores the remarkable advances in time-resolved fluorescence techniques and their role in a wide range of biological and clinical applications.
Broadly accessible, the book captures the state-of-the-art of fluorescence lifetime metrology and imaging and provides current perspectives on their applications to biomedical studies of intact tissues and medical diagnosis. The text introduces these techniques within the wider context of fluorescence spectroscopy and describes basic principles underlying current instrumentation for fluorescence lifetime imaging and metrology (FLIM). It also covers the wide range of methods, including single channel (point) spectroscopy, fluorescence lifetime imaging microscopy, and single- and multi-photon excitation.
Edited by pioneers in this field, with contributions from leading experts, the book includes an overview of complementary techniques that help researchers beginning FLIM research. It offers a comprehensive treatment of fundamental principles, instrumentation, analytical methods, and applications. It also provides an overview of the label-free contrast available from lifetime measurements of tissue autofluorescence and the prospects for exploiting this for clinical applications and biomedical research including drug discovery.Overview of Fluorescence Measurements and Concepts
Overview of Fluorescence Lifetime Imaging and Metrology,
Photophysics of Fluorescence
Tissue Fluorophores and Their Spectroscopic Characteristics
Principles of Fluorescence Lifetime Instrumentation
Pulse Sampling Technique
Single-Point Probes for Lifetime Spectroscopy: Time-Correlated Single-Photon Counting Technique
Optical Instrumentation Design for Fluorescence Lifetime Spectroscopy and Imaging
Fluorescence Lifetime Imaging Techniques: Frequency-Domain FLIM
Fluorescence Lifetime Imaging Techniques: Time-Gated Fluorescence Lifetime Imaging
Fluorescence Lifetime Imaging Techniques: Time-Correlated Single-Photon Counting
Analysis of Fluorescence Lifetime Data
The Phasor Approach to Fluorescence Lifetime Imaging: Exploiting Phasor Linear Properties
Analysis of Time-Domain Fluorescence Measurements Using Least-Squares Deconvolution
Global Analysis of FLIM-FRET data
Fluorescence Lifetime Imaging in Turbid Media
Tissue Autofluorescence Lifetime Spectroscopy and Imaging: Applications
Oncology Applications: Optical Diagnostics of CancerOncology Applications: Brain
Oncology Applications: Skin Cancer
Oncology Applications: Gastrointestinal Cancer
Oncology Applications: Intraoperative Diagnosis of Head and Neck Carcinoma
Fluorescence Lifetime Techniques in Cardiovascular Disease Diagnostics
Ophthalmic Applications of FLIM
Fluorescence Lifetime Imaging Applications in Tissue Engineering
Fluorescence Lifetime Imaging Based on Exogenous Probes
Tomographic Fluorescence Lifetime Imaging
Photosensitizers and PDT
Fluorescence Lifetime Imaging of Ions in Biological Tissues