Weissig V., Elbayoumi T., Olsen M. (Eds.) Cellular and Subcellular Nanotechnology: Methods and Protocols

Springer, 2013. — 368 p.In Cellular and Subcellular Nanotechnology: Methods and Protocols expert researchers in the field detail the most recent advances which have been made in utilizing the enormous potential of nanotechnology for probing, imaging and manipulating life on a cellular and subcellular level. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.Nanoparticle-GFP Chemical Nose Sensor for Cancer Cells Identification
A Method to Map Spatiotemporal pH Changes in a Multicellular Living Organism using a DNA Nanosensor
A Simple Method to Visualize and Assess the Integrity of Lysosomal Membrane in Mammalian Cells using a Fluorescent Dye
Gold Nanoparticle as a Marker for Precise Localization of Nano-objects within Intracellular Sub-Domains
Immunoisolation of Nanoparticles Containing Endocytic Vesicles for Drug Quantitation
Methods for Isolation and Identification of Nanoparticle-Containing Subcellular Compartments
Permeabilization of Cell Membrane for Delivery of Nano-objects to Cellular Sub-domains
A Method to Encapsulate Molecular Cargo within DNA Icosahedra
Delivery of Plasmid DNA to Mammalian Cells using Polymer-Gold Nanorod Assemblies
Lipophilic Formulated Gold Porphyrin Nanoparticles for Chemotherapy
Mitochondria-specific Nano-Emulsified Therapy for Myocardial Protection against Doxorubicin-induced Cardiotoxicity
Formation of Pit-Spanning Phospholipid Bilayers on Nanostructured Silicon Dioxide Surfaces for Studying Biological Membrane
Characterization of Nanoparticle – Lipid Membrane Interactions using QCM-D
Single-Cell Nanosurgery
Single Quantum Dot Imaging in Living Cells
Fabrication of Fluorescent Silica Nanoparticles with Aggregation-Induced Emission Luminogens for Cell Imaging
Monitoring the Degradation of Reduction Sensitive Gene Carriers with Fluorescence Spectroscopy and Flow Cytometry
Quantification of Intracellular Mitochondrial Displacements in Response to Nanomechanical Forces
Imaging Select Mammalian Organelles Using Fluorescent Microscopy: Application to Drug Delivery
Real-Time Particle Tracking for Studying Intracellular Trafficking of Pharmaceutical Nanocarriers
Interactions of Nanoparticles with Proteins -Determination of Equilibrium Constants
Tracing the Endocytic Pathways and Trafficking Kinetics of Cell Signaling Receptors using Single QD Nanoparticles
Cellular Internalization of Quantum Dots
Electrochemical Scanning Tunneling Microscopy and Spectroscopy for Single Molecule Investigation
Intracellular Delivery of Biologically Active Proteins with Peptide-based Carriers
Lipo-oligoarginine-based Intracellular Delivery
Fluorescent Spherical Monodisperse Silica Core-shell Nanoparticles with a Protein-binding Biofunctional Shell
Direct Quantification of PTD Transduction Using Real-Time Monitoring
Genotoxic Assessment of Carbon Nanotubes
Separation Science: Principles and Applications for the Analysis of Bionanoparticles by Asymmetrical Flow Field-Flow Fractionation (AF4)
Polymersomes-mediated Delivery of Fluorescent Probes for Targeted and Long Term Imaging in Live Cell Microscopy
Protocol for the Preparation of Stimuli-responsive Gold Nanoparticles Capped with Elastin-based Pentapeptides